The grapevine and wine microbiome : insights from high-throughput amplicon sequencing
Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Media
Abstract
From the time when microbial activity in wine fermentation was first demonstrated, the
microbial ecology of the vineyard, grape, and wine has been extensively investigated
using culture-based methods. However, the last 2 decades have been characterized
by an important change in the approaches used for microbial examination, due to
the introduction of DNA-based community fingerprinting methods such as DGGE,
SSCP, T-RFLP, and ARISA. These approaches allowed for the exploration of microbial
community structures without the need to cultivate, and have been extensively applied to
decipher the microbial populations associated with the grapevine as well as the microbial
dynamics throughout grape berry ripening and wine fermentation. These techniques are
well-established for the rapid more sensitive profiling of microbial communities; however,
they often do not provide direct taxonomic information and possess limited ability to
detect the presence of rare taxa and taxa with low abundance. Consequently, the past 5
years have seen an upsurge in the application of high-throughput sequencing methods
for the in-depth assessment of the grapevine and wine microbiome. Although a relatively
new approach in wine sciences, these methods reveal a considerably greater diversity
than previously reported, and identified several species that had not yet been reported.
The aim of the current review is to highlight the contribution of high-throughput next
generation sequencing and metagenomics approaches to vineyard microbial ecology
especially unraveling the influence of vineyard management practices on microbial
diversity.
Description
CITATION: Morgan, H. H., Du Toit, M. & Setati, M. E. 2017. The grapevine and wine microbiome : insights from high-throughput amplicon sequencing. Frontiers in Microbiology, 8:820, doi:10.3389/fmicb.2017.00820.
The original publication is available at https://www.frontiersin.org
The original publication is available at https://www.frontiersin.org
Keywords
Microbial diversity, Wine
Citation
Morgan, H. H., Du Toit, M. & Setati, M. E. 2017. The grapevine and wine microbiome : insights from high-throughput amplicon sequencing. Frontiers in Microbiology, 8:820, doi:10.3389/fmicb.2017.00820