Repression of sex4 and like sex four2 orthologs in potato increases tuber starch bound phosphate with concomitant alterations in starch properties physical

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dc.contributor.authorSamodien, Ebrahimen_ZA
dc.contributor.authorJewell, Jonathan F.en_ZA
dc.contributor.authorLoedolff, Biankeen_ZA
dc.contributor.authorOberlander, Kennethen_ZA
dc.contributor.authorGeorge, Gavin M.en_ZA
dc.contributor.authorZeeman, Samuel C.en_ZA
dc.contributor.authorDamberger, Fred F.en_ZA
dc.contributor.authorVan Der Vyver, Christellen_ZA
dc.contributor.authorKossmann, Jensen_ZA
dc.contributor.authorLloyd, James R.en_ZA
dc.date.accessioned2020-04-09T08:42:27Z
dc.date.available2020-04-09T08:42:27Z
dc.date.issued2018-07-23
dc.descriptionCITATION: Samodien, E., et al. 2018. Repression of sex4 and like sex four2 orthologs in potato increases tuber starch bound phosphate with concomitant alterations in starch properties physical. Frontiers in Plant Science, 9:1044. doi:10.3389/fpls.2018.01044.
dc.descriptionThe original publication is available at https://www.frontiersin.org
dc.description.abstractTo examine the roles of starch phosphatases in potatoes, transgenic lines were produced where orthologs of SEX4 and LIKE SEX FOUR2 (LSF2) were repressed using RNAi constructs. Although repression of either SEX4 or LSF2 inhibited leaf starch degradation, it had no effect on cold-induced sweetening in tubers. Starch amounts were unchanged in the tubers, but the amount of phosphate bound to the starch was significantly increased in all the lines, with phosphate bound at the C6 position of the glucosyl units increased in lines repressed in StSEX4 and in the C3 position in lines repressed in StLSF2 expression. This was accompanied by a reduction in starch granule size and an alteration in the constituent glucan chain lengths within the starch molecule, although no obvious alteration in granule morphology was observed. Starch from the transgenic lines contained fewer chains with a degree of polymerization (DP) of less than 17 and more with a DP between 17 and 38. There were also changes in the physical properties of the starches. Rapid viscoanalysis demonstrated that both the holding strength and the final viscosity of the high phosphate starches were increased indicating that the starches have increased swelling power due to an enhanced capacity for hydration.en_ZA
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fpls.2018.01044/full
dc.description.versionPublisher's version
dc.format.extent13 pages : illustrationsen_ZA
dc.identifier.citationSamodien, E., et al. 2018. Repression of sex4 and like sex four2 orthologs in potato increases tuber starch bound phosphate with concomitant alterations in starch properties physical. Frontiers in Plant Science, 9:1044, doi: 10.3389/fpls.2018.01044
dc.identifier.issn1664-462X (online)
dc.identifier.otherdoi: 10.3389/fpls.2018.01044
dc.identifier.urihttp://hdl.handle.net/10019.1/107675
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers Media
dc.rights.holderAuthors retain copyright
dc.subjectStarch cropsen_ZA
dc.subjectPhosphatasesen_ZA
dc.subjectPotatoes --- Biotechnologyen_ZA
dc.subjectPlants, Sex inen_ZA
dc.titleRepression of sex4 and like sex four2 orthologs in potato increases tuber starch bound phosphate with concomitant alterations in starch properties physicalen_ZA
dc.typeArticleen_ZA
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