Complex stability and dynamic subunit interchange modulates the disparate activities of the yeast moonlighting proteins Hal3 and Vhs3

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dc.contributor.authorAbrie, J. Alberten_ZA
dc.contributor.authorMolero, Cristinaen_ZA
dc.contributor.authorArino, Joaquinen_ZA
dc.contributor.authorStrauss, Ericken_ZA
dc.date.accessioned2016-11-28T08:47:49Z
dc.date.available2016-11-28T08:47:49Z
dc.date.issued2015-10
dc.descriptionCITATION: Abrie, J. A., Molero, C., Arino, J. & Strauss, E. 2015. Complex stability and dynamic subunit interchange modulates the disparate activities of the yeast moonlighting proteins Hal3 and Vhs3. Scientific Reports, 5:15774, doi:10.1038/srep15774.
dc.descriptionThe original publication is available at http://www.nature.com/srep
dc.description.abstractSaccharomyces cerevisiae Hal3 and Vhs3 are moonlighting proteins, acting both as inhibitors of the serine/threonine protein phosphatase Ppz1 and as subunits (together with Cab3) of the unique heterotrimeric phosphopantothenoylcysteine decarboxylase (PPCDC) enzyme of Hemiascomycetous yeast. Both these roles are essential: PPCDC catalyses the third step of coenzyme A biosynthesis, while Ppz1 inhibition is required for regulation of monovalent cation homeostasis. However, the mechanisms by which these proteins’ disparate activities are regulated are not well understood. The PPCDC domains (PDs) of Hal3, Vhs3 and Cab3 constitute the minimum requirement for these proteins to show both PPCDC activity and, in the case of Hal3 and Vhs3, to bind to Ppz1. Using these PD proteins as a model system to study the possibility of dynamic interchange between these roles, we provide evidence that Hal3 binds Ppz1 as a monomer (1:1 stoichiometry), requiring it to deoligomerize from its usual homo- and heterotrimeric states (the latter having PPCDC activity). This de-oligomerization is made possible by structural features that set Hal3 apart from Vhs3, increasing its ability to undergo monomer exchange. These findings suggest that oligomer interchange may be a significant factor in the functional regulation of these proteins and their various unrelated (moonlighting) functions.en_ZA
dc.description.urihttp://www.nature.com/articles/srep15774
dc.description.versionPublisher's version
dc.format.extent17 pages : illustrationsen_ZA
dc.identifier.citationAbrie, J. A., Molero, C., Arino, J. & Strauss, E. 2015. Complex stability and dynamic subunit interchange modulates the disparate activities of the yeast moonlighting proteins Hal3 and Vhs3. Scientific Reports, 5:15774, doi:10.1038/srep15774.
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/srep15774
dc.identifier.urihttp://hdl.handle.net/10019.1/99909
dc.language.isoen_ZAen_ZA
dc.publisherSpringer Nature
dc.rights.holderAuthors retain copyright
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.subjectYeast moonlighting proteinsen_ZA
dc.titleComplex stability and dynamic subunit interchange modulates the disparate activities of the yeast moonlighting proteins Hal3 and Vhs3en_ZA
dc.typeArticleen_ZA
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