Sex-dependent differences in phenobarbitone-induced oestradiol-2-hydroxylase activity in rat liver

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dc.contributor.authorTheron, C. N.en_ZA
dc.contributor.authorNeethling, A. C.en_ZA
dc.contributor.authorTaljaard, J. J. F.en_ZA
dc.date.accessioned2011-03-18T14:57:47Z
dc.date.available2011-03-18T14:57:47Z
dc.date.issued1981
dc.descriptionCITATION: Theron, C. N., Neethling, A. C. & Taljaard, J. J. F. 1981. Sex-dependent differences in phenobarbitone-induced oestradiol-2-hydroxylase activity in rat liver. South African Medical Journal, 60:279-282.
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.description.abstractOestradiol-2-hydroxylase (E2-OH) activity was measured in liver and brain microsomes of 6-8-week-old Wistar rats. Phenobarbitone (75 mg/kg daily for 3 days) significantly increased enzyme activity in the liver of males and females, but there were striking differences between the two sexes. In males the enzyme activity was increased by 37% over control values and in females by 200%. The total microsomal cytochrome P-450 content was increased by 75% in males and by 82% in females. The apparent Michaelis constant (Km) of E2-OH for 17β-oestradiol in untreated males (9.8 μM) and females (9.2 μM) did not differ significantly. Phenobarbitone treatment, however, tended to reduce the apparent Km in males (8.2 μM) and to increase it in females (18.7 μM). E2-OH activity was also detected in brain tissue of both sexes, but it was 50-200-fold lower than in the liver and was not increased by phenobarbitone.
dc.description.versionPublisher’s version
dc.format.extent4 pages
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/7646
dc.language.isoen
dc.publisherHealth & Medical Publishing Group
dc.rights.holderSouth African Medical Journal
dc.subjectEstradiolen_ZA
dc.titleSex-dependent differences in phenobarbitone-induced oestradiol-2-hydroxylase activity in rat liveren_ZA
dc.typeArticle
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