Chronic prosopis glandulosa treatment blunts neutrophil infiltration and enhances muscle repair after contusion injury
Date
2015-01
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
The current treatment options for soft tissue injuries remain suboptimal and often
result in delayed/incomplete recovery of damaged muscle. The current study aimed to
evaluate the effects of oral Prosopis glandulosa treatment on inflammation and regeneration
in skeletal muscle after contusion injury, in comparison to a conventional treatment.
The gastrocnemius muscle of rats was subjected to mass-drop injury and muscle samples
collected after 1-, 3 h, 1- and 7 days post-injury. Rats were treated with P. glandulosa
(100 mg/kg/day) either for 8 weeks prior to injury (up until day 7 post-injury), only
post-injury, or with topically applied diclofenac post-injury (0.57 mg/kg). Neutrophil
(His48-positive) and macrophage (F4/80-positive) infiltration was assessed by means of
immunohistochemistry. Indicators of muscle satellite cell proliferation (ADAM12) and
regeneration (desmin) were used to evaluate muscle repair. Chronic P. glandulosa and
diclofenac treatment (p < 0.0001) was associated with suppression of the neutrophil response
to contusion injury, however only chronic P. glandulosa treatment facilitated more effective
muscle recovery (increased ADAM12 (p < 0.05) and desmin (p < 0.001) expression), while
diclofenac treatment had inhibitory effects on repair, despite effective inhibition of
neutrophil response. Data indicates that P. glandulosa treatment results in more effective
muscle repair after contusion.
Description
CITATION: George, C., et al. 2015. Chronic prosopis glandulosa treatment blunts neutrophil infiltration and enhances muscle repair after contusion injury. Nutrients, 7(2):815-830, doi:10.3390/nu7020815.
The original publication is available at https://www.mdpi.com
Publication of this article was funded by the Stellenbosch University Open Access Fund.
The original publication is available at https://www.mdpi.com
Publication of this article was funded by the Stellenbosch University Open Access Fund.
Keywords
Fabaceae, Inflammation -- Treatment, Mass-drop injury, Nonsteroidal anti-inflammatory agents, Musculoskeletal system -- Regeneration
Citation
George, C., et al. 2015. Chronic prosopis glandulosa treatment blunts neutrophil infiltration and enhances muscle repair after contusion injury. Nutrients, 7(2):815-830, doi:10.3390/nu7020815