Polymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes : a South African perspective

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dc.contributor.authorLaaks, Debbieen_ZA
dc.contributor.authorSmit, Derrick Peteren_ZA
dc.contributor.authorHarvey, Justinen_ZA
dc.date.accessioned2017-02-15T13:05:25Z
dc.date.available2017-02-15T13:05:25Z
dc.date.issued2015
dc.descriptionCITATION: Laaks, D., Smit, D. P. & Harvey, J. Polymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes : a South African perspective. African health sciences, 15(3):748–754, doi:10.4314/ahs.v15i3.7.
dc.descriptionThe original publication is available at http://www.ajol.info/
dc.description.abstractObjective: To analyse aqueous polymerase chain reaction (PCR) results in patients diagnosed with undifferentiated uveitis and determine prevalence of herpesviridae in non-uveitic patients undergoing routine cataract extraction. Design: Retrospective comparative case series and prospective cross-sectional study. Subjects: 72 patients with idiopathic uveitis and 57 surgical patients. Methods: Diagnostic aqueous paracentesis with PCR testing for 6 herpes viridae in uveitic patients. Anterior chamber paracentesis immediately pre-operative in the prospective arm, with PCR testing. Results: In the retrospective review we had a 47.2% positive PCR yield. Data analysis revealed a statistically significant correlation between a positive yield and being HIV+ (p=0.018); between an EBV+ yield and being HIV+ (p= 0.026) and a CMV+ result and being HIV+ (p=0.032). Posterior uveitis (p=0.014) and symptoms <30 days (p= 0.0014) had a statistically significant yield. In the prospective arm of the study: all 57 patients were HIV- and all aqueous samples were negative for the 6 herpesviridae. Conclusion: We recommend PCR testing for Herpesviridae as a safe second line test for patients with undifferentiated uveitis. We were unable to establish prevalence and suggest that the idea of a commensal herpes virus is unlikely if the blood-ocular barrier is intacten_ZA
dc.description.urihttp://www.ajol.info/index.php/ahs/article/view/121827
dc.description.versionPost print
dc.description.versionMasters
dc.format.extent7 pagesen_ZA
dc.identifier.citationLaaks, D., Smit, D. P. & Harvey, J. Polymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes : a South African perspective. African health sciences, 15(3):748–754, doi:10.4314/ahs.v15i3.7
dc.identifier.issn1729-0503 (online)
dc.identifier.issn1680-6905 (print)
dc.identifier.otherdoi:10.4314/ahs.v15i3.7
dc.identifier.urihttp://hdl.handle.net/10019.1/100631
dc.language.isoen_ZAen_ZA
dc.publisherMakerere University, Faculty of Medicine
dc.rights.holderAuthors retain copyright
dc.subjectPolymerase chain reactionen_ZA
dc.subjectCataract -- Surgeryen_ZA
dc.subjectUveitisen_ZA
dc.subjectHerpesvirus infections
dc.titlePolymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes : a South African perspectiveen_ZA
dc.typeArticleen_ZA
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