dc.contributor.author	Young, Philip R.
dc.contributor.author	Lashbrooke, Justin G.
dc.contributor.author	Alexandersson, Erik
dc.contributor.author	Jacobson, Dan
dc.contributor.author	Moser, Claudio
dc.contributor.author	Velasco, Riccardo
dc.contributor.author	Vivier, Melané A.
dc.date.accessioned	2012-12-14T06:47:48Z
dc.date.available	2012-12-14T06:47:48Z
dc.date.issued	2012-06
dc.date.updated	2012-10-30T12:07:41Z
dc.description	The original publication is available at http://www.biomedcentral.com/1471-2164/13/243	en_ZA
dc.description	Publication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstract	Abstract Background Carotenoids are a heterogeneous group of plant isoprenoids primarily involved in photosynthesis. In plants the cleavage of carotenoids leads to the formation of the phytohormones abscisic acid and strigolactone, and C13-norisoprenoids involved in the characteristic flavour and aroma compounds in flowers and fruits and are of specific importance in the varietal character of grapes and wine. This work extends the previous reports of carotenoid gene expression and photosynthetic pigment analysis by providing an up-to-date pathway analysis and an important framework for the analysis of carotenoid metabolic pathways in grapevine. Results Comparative genomics was used to identify 42 genes putatively involved in carotenoid biosynthesis/catabolism in grapevine. The genes are distributed on 16 of the 19 chromosomes and have been localised to the physical map of the heterozygous ENTAV115 grapevine sequence. Nine of the genes occur as single copies whereas the rest of the carotenoid metabolic genes have more than one paralogue. The cDNA copies of eleven corresponding genes from Vitis vinifera L. cv. Pinotage were characterised, and four where shown to be functional. Microarrays provided expression profiles of 39 accessions in the metabolic pathway during three berry developmental stages in Sauvignon blanc, whereas an optimised HPLC analysis provided the concentrations of individual carotenoids. This provides evidence of the functioning of the lutein epoxide cycle and the respective genes in grapevine. Similarly, orthologues of genes leading to the formation of strigolactone involved in shoot branching inhibition were identified: CCD7, CCD8 and MAX1. Moreover, the isoforms typically have different expression patterns, confirming the complex regulation of the pathway. Of particular interest is the expression pattern of the three VvNCEDs: Our results support previous findings that VvNCED3 is likely the isoform linked to ABA content in berries. Conclusions The carotenoid metabolic pathway is well characterised, and the genes and enzymes have been studied in a number of plants. The study of the 42 carotenoid pathway genes of grapevine showed that they share a high degree of similarity with other eudicots. Expression and pigment profiling of developing berries provided insights into the most complete grapevine carotenoid pathway representation. This study represents an important reference study for further characterisation of carotenoid biosynthesis and catabolism in grapevine.	en_ZA
dc.description.version	Peer Reviewed	en_ZA
dc.format.extent	17 p. : ill.
dc.identifier.citation	Young, P. R. et al. 2012. The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L. BMC Genomics, 13(1), 243, doi:10.1186/1471-2164-13-243.	en_ZA
dc.identifier.issn	doi:10.1186/1471-2164-13-243
dc.identifier.issn	1471-2164 (online)
dc.identifier.uri	http://hdl.handle.net/10019.1/71993
dc.language.iso	en_ZA	en_ZA
dc.language.rfc3066	en
dc.publisher	BioMed Central	en_ZA
dc.rights.holder	Philip R Young et al.; licensee BioMed Central Ltd.	en_ZA
dc.subject	Grapevine -- Carotenoid biosynthesis	en_ZA
dc.subject	Plants -- Photosynthesis	en_ZA
dc.subject	Grapevines -- Photosynthetic pigment analysis	en_ZA
dc.title	The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L	en_ZA
dc.type	Article	en_ZA

The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L

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