The paracrine effects of fibroblasts on Doxorubicin-treated breast cancer cells

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dc.contributor.authorCarla, Fourieen_ZA
dc.contributor.authorDavis, Tanjaen_ZA
dc.contributor.authorKriel, Jurgenen_ZA
dc.contributor.authorEngelbrecht, Anna-Marten_ZA
dc.date.accessioned2021-09-17T06:17:31Z
dc.date.available2021-09-17T06:17:31Z
dc.date.issued2019
dc.descriptionCITATION: Carla, F., et al. 2019. The paracrine effects of fibroblasts on Doxorubicin-treated breast cancer cells. Experimental Cell Research, 381(2):280-287. doi:10.1016/j.yexcr.2019.05.020
dc.descriptionThe original publication is available at https://www.sciencedirect.com/journal/experimental-cell-research
dc.descriptionThe Doctoral/Master’s degree for this article is available at [http://hdl.handle.net/10019.1/106181]
dc.description.abstractBreast cancer is frequently diagnosed in women and poses a major health problem throughout the world. Currently, the unresponsiveness of cancer cells to chemotherapeutics is a major concern. During chemotherapeutic treatment with Doxorubicin, neighbouring cells in the tumor microenvironment are also damaged. Depending on the concentration of Doxorubicin, apoptotic or senescent fibroblasts in the tumor microenvironment can then secrete a variety of bioactive molecules which promote tumor growth, metastasis and drug resistance. Mouse embryonic fibroblasts (MEFs) were treated with Doxorubicin to induce apoptosis and senescence respectively. Conditioned media was collected from the MEFs and was used to assess the paracrine effects between fibroblasts and E0771 murine breast cancer cells. Senescent fibroblasts significantly increased cell viability in E0771 cells following Doxorubicin treatment by activating Akt and ERK. Autophagy contributed to cancer cell death and not to treatment resistance in breast cancer cells. Our results highlight the complexity of the tumor microenvironment where chemotherapeutic agents such as Doxorubicin can induce significant changes fibroblasts which can affect tumor growth via the secretion of paracrine factors. Here we have demonstrated that those secreted paracrine factors enhance breast cancer growth and induce therapeutic resistance through the evasion of apoptotic cell death.en_ZA
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0014482719302617?via%3Dihub
dc.description.versionPublisher's version
dc.format.extent8 pages : illustrationsen_ZA
dc.identifier.citationCarla, F., et al. 2019. The paracrine effects of fibroblasts on Doxorubicin-treated breast cancer cells. Experimental Cell Research, 381(2):280-287. doi:10.1016/j.yexcr.2019.05.020
dc.identifier.issn0014-4827 (print)
dc.identifier.otherdoi:10.1016/j.yexcr.2019.05.020
dc.identifier.urihttp://hdl.handle.net/10019.1/123038
dc.language.isoen_ZAen_ZA
dc.publisherElsevier
dc.rights.holderElsevier
dc.subjectApoptosis -- Physiologyen_ZA
dc.subjectBreast -- Cancer -- Chemotherapyen_ZA
dc.subjectDoxorubicin -- Chemotherapyen_ZA
dc.subjectFibroblastsen_ZA
dc.subjectSenescenceen_ZA
dc.subjectBreast cancer -- Treatment resistanceen_ZA
dc.subjectApoptosis -- Effect of drugs onen_ZA
dc.subjectDrug resistance in cancer cellsen_ZA
dc.titleThe paracrine effects of fibroblasts on Doxorubicin-treated breast cancer cellsen_ZA
dc.typeArticleen_ZA
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