Deriving a grain hardness calibration for Southern and Western Cape ground wheat samples by means of Fourier transform near infrared (FT-NIR) spectroscopy
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2001
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ENGLISH ABSTRACT: The bread wheat hardness scenario among the Southern and Western Cape cultivars was investigated by deriving a FT-NIR hardness calibration for 198 ground bread wheat samples and using the particle size index (PSI) test as reference method. Particle size index results indicate that the investigated bread wheat cultivars a have very wide hardness range of 37.05 - 60.50%. The influence of genotype, location, protein content and moisture content on wheat hardness was investigated by means of ANOVA, Student's t-test, correlation analy sis and regression analysis. Significant differences (P 0.05) in hardness were found for culitvars, while a pos < itive linear relationship with protein content and a negative linear relationship with moisture content exist. The wheat samples were subsequently presented to the spectrophotometer in individual borosilicate-glass vials. A FT-NIR calibration was derived by performing a PLS regression on baseline-corrected spectra and the model was validated using an independent validation. Excellent results were achieved with an SEP, RMSEP and r of 2.13%,4.53% and 0.89, respectively.
AFRIKAANSE OPSOMMING: Die broodkoringhardheid scenario onder Suid- en Wes-Kaap kultivars is ondersoek deur 'n. FT-NIR hardheid kalibrasie vir 198 gemaalde broodkoringmonsterste ontwikkel waar die partikelgrootte-indeks (PGI) metode as verwysingsmetode gebruik is. Volgens die PGI resultate het die betrokke broodkoringkultivars 'n wye hard heidsreeks van 37.05 - 60.50% getoon, wat daarop dui dat die broodkoringklas in die Suid- en Wes-Kaap 'n besonderse wye reeks hardhede bevat. Die invloed van genotipe, lokaliteite, prote"ien- en voginhoud op graan hardheid is met behulp va!"! statistiese ontledings ondersoek. Betekenisvolle verskille in hardheid is gevind vir· monsters van verskillende genotipes (P :s; 0.05). Hardheid toon verder 'n positiewe liniere verband met prote"ien en 'n negatiewe liniere verband met voginhoud. Monsters is vervolgens aan die spektrofotometer blootgestel in individuele borosilikaat-glasflessies. 'n FT-NIR kalibrasie is ontwikkel deur die som van die kleinste kwadrate (PLS) regressie uit te voer op 'n basislyn-gekorrigeerde spektra. Die model is gevalideer met behulp van onaf hanklike validasieprosedures. Goeie resultate is verkry met 'n standaardfout van ·voorspelling (RMSEP), 'n standaardfout van voorspelling gekorrigeer vir oorhelling (SEP) en 'n korrelasiekoeffisiemt (r) van onderskeidelik 4.53%,2.13% en 0.89.
ENGLISH ABSTRACT: The bread wheat hardness scenario among the Southern and Western Cape cultivars was investigated by deriving a FT-NIR hardness calibration for 198 ground bread wheat samples and using the particle size index (PSI) test as reference method. Particle size index results indicate that the investigated bread wheat cultivars a have very wide hardness range of 37.05 - 60.50%. The influence of genotype, location, protein content and moisture content on wheat hardness was investigated by means of ANOVA, Student's t-test, correlation analy sis and regression analysis. Significant differences (P 0.05) in hardness were found for culitvars, while a pos < itive linear relationship with protein content and a negative linear relationship with moisture content exist. The wheat samples were subsequently presented to the spectrophotometer in individual borosilicate-glass vials. A FT-NIR calibration was derived by performing a PLS regression on baseline-corrected spectra and the model was validated using an independent validation. Excellent results were achieved with an SEP, RMSEP and r of 2.13%,4.53% and 0.89, respectively.
AFRIKAANSE OPSOMMING: Die broodkoringhardheid scenario onder Suid- en Wes-Kaap kultivars is ondersoek deur 'n. FT-NIR hardheid kalibrasie vir 198 gemaalde broodkoringmonsterste ontwikkel waar die partikelgrootte-indeks (PGI) metode as verwysingsmetode gebruik is. Volgens die PGI resultate het die betrokke broodkoringkultivars 'n wye hard heidsreeks van 37.05 - 60.50% getoon, wat daarop dui dat die broodkoringklas in die Suid- en Wes-Kaap 'n besonderse wye reeks hardhede bevat. Die invloed van genotipe, lokaliteite, prote"ien- en voginhoud op graan hardheid is met behulp va!"! statistiese ontledings ondersoek. Betekenisvolle verskille in hardheid is gevind vir· monsters van verskillende genotipes (P :s; 0.05). Hardheid toon verder 'n positiewe liniere verband met prote"ien en 'n negatiewe liniere verband met voginhoud. Monsters is vervolgens aan die spektrofotometer blootgestel in individuele borosilikaat-glasflessies. 'n FT-NIR kalibrasie is ontwikkel deur die som van die kleinste kwadrate (PLS) regressie uit te voer op 'n basislyn-gekorrigeerde spektra. Die model is gevalideer met behulp van onaf hanklike validasieprosedures. Goeie resultate is verkry met 'n standaardfout van ·voorspelling (RMSEP), 'n standaardfout van voorspelling gekorrigeer vir oorhelling (SEP) en 'n korrelasiekoeffisiemt (r) van onderskeidelik 4.53%,2.13% en 0.89.
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CITATION: Manley, M., Van Zyl, L. & Osborne, B. 2001. Deriving a grain hardness calibration for Southern and Western Cape ground wheat samples by means of Fourier transform near infrared (FT-NIR) spectroscopy. South African Journal of Plant and Soil, 18(2).
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Citation
Manley, M., Van Zyl, L. & Osborne, B. 2001. Deriving a grain hardness calibration for Southern and Western Cape ground wheat samples by means of Fourier transform near infrared (FT-NIR) spectroscopy. South African Journal of Plant and Soil, 18(2).