Proteomics : a subcellular look at spermatozoa

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dc.contributor.authorDu Plessis, Stefan S.
dc.contributor.authorKashou, Anthony H.
dc.contributor.authorBenjamin, David J.
dc.contributor.authorYadav, Satya P.
dc.contributor.authorAgarwal, Ashok
dc.date.accessioned2011-04-06T08:12:29Z
dc.date.available2011-04-06T08:12:29Z
dc.date.issued2011-03
dc.date.updated2011-04-06T05:08:36Z
dc.description.abstractAbstract. Background. Male-factor infertility presents a vexing problem for many reproductively active couples. Many studies have focused on abnormal sperm parameters. Recent advances in proteomic techniques, especially in mass spectrometry, have aided in the study of sperm and more specifically, sperm proteins. The aim of this study was to review the current literature on the various proteomic techniques, and their usefulness in diagnosing sperm dysfunction and potential applications in the clinical setting. Methods Review of PubMed database. Key words: spermatozoa, proteomics, protein, proteome, 2D-PAGE, mass spectrometry. Results. Recently employed proteomic methods, such as two-dimensional polyacrylamide gel electrophoresis, mass spectrometry, and differential in gel electrophoresis, have identified numerous sperm-specific proteins. They also have provided a further understanding of protein function involved in sperm processes and for the differentiation between normal and abnormal states. In addition, studies on the sperm proteome have demonstrated the importance of post-translational modifications, and their ability to bring about physiological changes in sperm function. No longer do researchers believe that in order for them to elucidate the biochemical functions of genes, mere knowledge of the human genome sequence is sufficient. Moreover, a greater understanding of the physiological function of every protein in the tissue-specific proteome is essential in order to unravel the biological display of the human genome. Conclusion Recent advances in proteomic techniques have provided insight into sperm function and dysfunction. Several multidimensional separation techniques can be utilized to identify and characterize spermatozoa. Future developments in bioinformatics can further assist researchers in understanding the vast amount of data collected in proteomic studies. Moreover, such advances in proteomics may help to decipher metabolites which can act as biomarkers in the detection of sperm impairments and to potentially develop treatment for infertile couples. Further comprehensive studies on sperm-specific proteome, mechanisms of protein function and its proteolytic regulation, biomarkers and functional pathways, such as oxidative-stress induced mechanisms, will provide better insight into physiological functions of the spermatozoa. Large-scale proteomic studies using purified protein assays will eventually lead to the development of novel biomarkers that may allow for detection of disease states, genetic abnormalities, and risk factors for male infertility. Ultimately, these biomarkers will allow for a better diagnosis of sperm dysfunction and aid in drug development.en_ZA
dc.description.sponsorshipCentre for Reproductive Medicine, Cleveland Clinic
dc.description.versionPeer Reviewed
dc.format.extent12 p. : ill.
dc.identifier.citationDu Plessis, SS, Kashou, AH, Benjamin, DJ, Yadav, SP & Agarwal, A 2011, 'Proteomics: a subcellular look at spermatozoa', Reproductive Biology and Endocrinology, 9(1):36.en_ZA
dc.identifier.issn1477-7827
dc.identifier.otherhttp://dx.doi.org/10.1186/1477-7827-9-36
dc.identifier.urihttp://hdl.handle.net/10019.1/8476
dc.language.isoen_USen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderDu Plessis et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectSpermatozoaen_ZA
dc.subjectProteomicsen_ZA
dc.subjectProteinen_ZA
dc.subjectProteomeen_ZA
dc.subjectinfertility, Male -- Diagnosisen_ZA
dc.subjectproteomics -- Techniquesen_ZA
dc.subjectSpermatozoa -- Motility -- Treatmenten_za
dc.titleProteomics : a subcellular look at spermatozoaen_ZA
dc.typeArticleen_ZA
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