NanoHIV: a bioinformatics pipeline for producing accurate, near full-length HIV proviral genomes sequenced using the Oxford Nanopore technology

Files
imogen_nano_2021.pdf(735.58 KB)
Date
2021-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
HIV-1 proviral single-genome sequencing by limiting-dilution polymerase chain reaction (PCR) amplification is important for differentiating the sequence-intact from defective proviruses that persist during antiretroviral therapy (ART). Intact proviruses may rebound if ART is interrupted and are the barrier to an HIV cure. Oxford Nanopore Technologies (ONT) sequencing offers a promising, cost-effective approach to the sequencing of long amplicons such as near full-length HIV-1 proviruses, but the high diversity of HIV-1 and the ONT sequencing error render analysis of the generated data difficult. NanoHIV is a new tool that uses an iterative consensus generation approach to construct accurate, near full-length HIV-1 proviral single-genome sequences from ONT data. To validate the approach, single-genome sequences generated using NanoHIV consensus building were compared to Illumina® consensus building of the same nine single-genome near full-length amplicons and an average agreement of 99.4% was found between the two sequencing approaches.
Description
CITATION: Wright, I.A.; Delaney, K.E.; Katusiime, M.G.K.; Botha, J.C.; Engelbrecht, S.; Kearney,M.F.; van Zyl, G.U. 2021. NanoHIV: A Bioinformatics Pipeline for Producing Accurate, Near Full-Length HIV Proviral Genomes Sequenced Using the Oxford Nanopore Technology. Cells , 10, 2577. doi.10.3390/cells10102577
The original publication is available at: mdpi.com
Keywords
HIV, Nanopore, Proviral, Consensus, Single-genome
Citation
Wright, I.A.; Delaney, K.E.; Katusiime, M.G.K.; Botha, J.C.; Engelbrecht, S.; Kearney,M.F.; van Zyl, G.U. 2021. NanoHIV: A Bioinformatics Pipeline for Producing Accurate, Near Full-Length HIV Proviral Genomes Sequenced Using the Oxford Nanopore Technology. Cells , 10, 2577. doi.10.3390/cells10102577
URI
http://hdl.handle.net/10019.1/126904
Collections
Research Articles (Medical Virology)