A sensitive urinary lipoarabinomannan test for tuberculosis
Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Abstract
We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial
lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration
step. We have now developed an easy to perform test based on a magnetic
immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM
in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold
compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically
verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and
the specificity 100%. This is in stark positive contrast to a range of studies using available
commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIVnegative
TB patients was very low.
Description
CITATION: Hamasur, B., et al. 2015. A sensitive urinary lipoarabinomannan test for tuberculosis. PLoS
ONE, 10(4):1-11, doi:10.1371/journal.pone.0123457.
The original publication is available at http://journals.plos.org/plosone
The original publication is available at http://journals.plos.org/plosone
Keywords
Tuberculosis -- Diagnosis, Lipoarabinomannan, Urine -- Examination, Microbiological assay, Monoclonal antibodies -- Diagnostic use
Citation
Hamasur, B., et al. 2015. A sensitive urinary lipoarabinomannan test for tuberculosis. PLoS
ONE, 10(4):1-11, doi:10.1371/journal.pone.0123457