Prevalence and trends of staphylococcus aureus bacteraemia in hospitalized patients in South Africa, 2010 to 2012 : laboratory- based surveillance mapping of antimicrobial resistance and molecular epidemiology

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dc.contributor.authorPerovic, Olgaen_ZA
dc.contributor.authorIyaloo, Samanthaen_ZA
dc.contributor.authorKularatne, Ranminien_ZA
dc.contributor.authorLowman, Warrenen_ZA
dc.contributor.authorBosman, Nomaen_ZA
dc.contributor.authorWadula, Jeannetteen_ZA
dc.contributor.authorSeetharam, Sharonaen_ZA
dc.contributor.authorDuse, Adrianoen_ZA
dc.contributor.authorMbelle, Nontombien_ZA
dc.contributor.authorBamford, Colleenen_ZA
dc.contributor.authorDawood, Halimaen_ZA
dc.contributor.authorMahabeer, Yesholataen_ZA
dc.contributor.authorBhola, Prathnaen_ZA
dc.contributor.authorAbrahams, Shareefen_ZA
dc.contributor.authorSingh-Moodley, Ashikaen_ZA
dc.date.accessioned2016-08-26T11:55:25Z
dc.date.available2016-08-26T11:55:25Z
dc.date.issued2015-12en_ZA
dc.descriptionCITATION: Perovic, O., et al. 2015. Prevalence and trends of staphylococcus aureus bacteraemia in hospitalized patients in South Africa, 2010 to 2012 : laboratory- based surveillance mapping of antimicrobial resistance and molecular epidemiology. PLoS ONE, 10(12):1-14, doi:10.1371/journal.pone.0145429.en_ZA
dc.descriptionThe original publication is available at http://journals.plos.org/plosoneen_ZA
dc.description.abstractIntroduction: We aimed to obtain an in-depth understanding on recent antimicrobial resistance trends and molecular epidemiology trends of S. aureus bacteraemia (SAB). Methods: Thirteen academic centres in South Africa were included from June 2010 until July 2012. S. aureus susceptibility testing was performed on the MicroScan Walkaway. Real-time PCR using the LightCycler 480 II was done for mecA and nuc. SCCmec and spa-typing were finalized with conventional PCR. We selected one isolate per common spa type per province for multilocus sequence typing (MLST). Results: S. aureus from 2709 patients were included, and 1231 (46%) were resistant to methicillin, with a significant decline over the three-year period (p-value = 0.003). Geographical distribution of MRSA was significantly higher in Gauteng compared to the other provinces (P<0.001). Children <5 years were significantly associated with MRSA with higher rates compared to all other age groups (P = 0.01). The most prevalent SCCmec type was SCCmec type III (531 [41%]) followed by type IV (402 [31%]). Spa-typing discovered 47 different spa-types. The five (87%) most common spa-types were t037, t1257, t045, t064 and t012. Based on MLST, the commonest was ST612 clonal complex (CC8) (n = 7) followed by ST5 (CC5) (n = 4), ST36 (CC30) (n = 4) and ST239 (CC8) (n = 3). Conclusions: MRSA rate is high in South Africa. Majority of the isolates were classified as SCCmec type III (41%) and type IV (31%), which are typically associated with hospital and community- acquired infections, respectively. Overall, this study reveals the presence of a variety of hospital-acquired MRSA clones in South Africa dominance of few clones, spa 037 and 1257. Monitoring trends in resistance and molecular typing is recommended to detect changing epidemiological trends in AMR patterns of SAB.en_ZA
dc.description.versionPublisher's version
dc.format.extent14 pagesen_ZA
dc.identifier.citationPerovic, O., et al. 2015. Prevalence and trends of staphylococcus aureus bacteraemia in hospitalized patients in South Africa, 2010 to 2012 : laboratory- based surveillance mapping of antimicrobial resistance and molecular epidemiology. PLoS ONE, 10(12):1-14, doi:10.1371/journal.pone.0145429en_ZA
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0145429
dc.identifier.urihttp://hdl.handle.net/10019.1/99484
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectStaphylococcus aureus bacteraemiaen_ZA
dc.subjectMolecular epidemiologyen_ZA
dc.subjectAntimicrobial resistanceen_ZA
dc.titlePrevalence and trends of staphylococcus aureus bacteraemia in hospitalized patients in South Africa, 2010 to 2012 : laboratory- based surveillance mapping of antimicrobial resistance and molecular epidemiologyen_ZA
dc.typeArticleen_ZA
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