Diagnostic potential of novel salivary host biomarkers as candidates for the immunological diagnosis of tuberculosis disease and monitoring of tuberculosis treatment response

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dc.contributor.authorJacobs, Ruschcaen_ZA
dc.contributor.authorMaasdorp, Eliznaen_ZA
dc.contributor.authorMalherbe, Stephanusen_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorVan Der Spuy, Gianen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorChegou, Novel N.en_ZA
dc.date.accessioned2016-08-10T06:28:43Z
dc.date.available2016-08-10T06:28:43Z
dc.date.issued2016-08-03
dc.descriptionCITATION: Jacobs, R., et al. 2016. Diagnostic potential of novel salivary host biomarkers as candidates for the immunological diagnosis of tuberculosis disease and monitoring of tuberculosis treatment response. PLoS ONE, 11(8):1-13, doi:10.1371/journal.pone.0160546.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractBackground: There is an urgent need for new tools for the early diagnosis of TB disease and monitoring of the response to treatment, especially in resource-constrained settings. We investigated the usefulness of host markers detected in saliva as candidate biomarkers for the immunological diagnosis of TB disease and monitoring of treatment response. Methods: We prospectively collected saliva samples from 51 individuals that presented with signs and symptoms suggestive of TB disease at a health centre in Cape Town, South Africa, prior to the establishment of a clinical diagnosis. Patients were later classified as having TB disease or other respiratory disease (ORD), using a combination of clinical, radiological and laboratory findings. We evaluated the concentrations of 69 host markers in saliva samples using a multiplex cytokine platform, and assessed the diagnostic potentials of these markers by receiver operator characteristics (ROC) curve analysis, and general discriminant analysis. Results: Out of the 51 study participants, 18 (35.4%) were diagnosed with TB disease and 12 (23.5%) were HIV infected. Only two of the 69 host markers that were evaluated (IL-16 and IL-23) diagnosed TB disease individually with area under the ROC curve ≥0.70. A five-marker biosignature comprising of IL-1β, IL-23, ECM-1, HCC1 and fibrinogen diagnosed TB disease with a sensitivity of 88.9% (95% CI,76.7–99.9%) and specificity of 89.7% (95% CI, 60.4–96.6%) after leave-one-out cross validation, regardless of HIV infection status. Eight-marker biosignatures performed with a sensitivity of 100% (95% CI, 83.2–100%) and specificity of 95% (95% CI, 68.1–99.9%) in the absence of HIV infection. Furthermore, the concentrations of 11 of the markers changed during treatment, indicating that they may be useful in monitoring of TB treatment response. Conclusion: We have identified novel salivary biosignatures which may be useful in the diagnosis of TB disease and monitoring of the response to TB treatment. Our findings require further validation in larger studies before these biosignatures could be considered for point-of-care screening test development.en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0160546
dc.description.versionPublisher's version
dc.format.extent13 pagesen_ZA
dc.identifier.citationJacobs, R., et al. 2016. Diagnostic potential of novel salivary host biomarkers as candidates for the immunological diagnosis of tuberculosis disease and monitoring of tuberculosis treatment response. PLoS ONE, 11(8): 1-13, doi:10.1371/journal.pone.0160546
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0160546
dc.identifier.urihttp://hdl.handle.net/10019.1/99346
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTuberculosisen_ZA
dc.subjectImmunological diagnosisen_ZA
dc.subjectBiomarkersen_ZA
dc.subjectTherapeutic drug monitoringen_ZA
dc.subjectTuberculosis -- Treatmenten_ZA
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.titleDiagnostic potential of novel salivary host biomarkers as candidates for the immunological diagnosis of tuberculosis disease and monitoring of tuberculosis treatment responseen_ZA
dc.typeArticleen_ZA
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