Department of Agronomy
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- ItemAspects of honeybush tea (Cyclopia species) propagation(Stellenbosch : Stellenbosch University, 2008-12) Mbangcolo, Mongezi Morrison; Reinten, E.; Agenbag, G. A.; Stellenbosch University. Faculty of AgriSciences. Dept. of Agronomy.Honeybush (Cyclopia spp. Fabaceae) is indigenous to the fynbos botanical biome of the Eastern and Western Cape of South Africa. The increase in the international demand for honeybush tea for health benefits, concern over exploitation of wild populations and the lack of published agronomic information necessitated this study to evaluate different aspects of honeybush propagation. The main objectives of this study were to evaluate the effect of species and cutting position on rooting of cuttings of Cyclopia species using different rooting hormones, to evaluate the effect of an organic plant fertilizer and cutting position on growth and establishment of rooted cuttings and to study the influence of different seed pre-treatments on germination of Cyclopia species. Terminal and sub-terminal cuttings of C. intermedia and C. genistoides treated with different rooting hormones were rooted under day/night temperature controlled glasshouse conditions. Intermittent mist was used as means of moisture supply to the cuttings for 45-60 seconds daily every 30 minutes. C. genistoides rooted significantly better compared to C. intermedia as measured by rooting percentage, number of roots per cutting, length of longest root and mean root length during the summer season. The cutting position had a significant effect on rooting of the cuttings in summer compared to winter and spring season. The interactive effect of species, treatment and cutting position resulted into 86% of rooting in summer from the terminal cuttings of C. genistoides, while only 4% was recorded as the highest rooting percentage in both winter and spring seasons. The highest number of roots and the greatest root length per cutting were obtained with 2 and 4 g L-1 IBA from terminal cuttings of C. genistoides and these hormone concentrations were not significantly different to each other. To evaluate the effect of an organic plant fertilizer and cutting position on plant growth and establishment, rooted cuttings of two Cyclopia species (C. intermedia and C. genistoides) from two cutting positions (terminal and sub-terminal) were transferred to pots (576 cm3) and treated with Nitrosol fertilizer at application rates of 3.33 ml.L-1, 1.67 ml.L-1 and 0 ml.L-1 (control). Cyclopia plantlets were uniformly inoculated once with a symbiotic Rhizobium bacteria to improve the formation of nodules. Nitrosol® at 3.33 ml.L-1 significantly affected fresh and dry plant weight, fresh and dry root weight, number of shoots and nodules per plant compared to either 1.67 ml.L-1 or the control. Relative to species, C. genistoides performed better in terms of fresh and dry plant weight, fresh and dry root weight, and number of shoots and nodules per plant compared to C. intermedia. The origin of the cutting position did not significantly affect the above mentioned parameters. Plant mineral analysis revealed that most of the essential elements increased with increasing Nitrosol® application rates, with C. genistoides having higher levels of mineral elements than C. intermedia. This could be an indication of the differences between the two species in terms of nutrient uptake, utilization and distribution within the plant tissues. In the germination studies, seeds obtained from different seed sources of Cyclopia species were subjected to different pre-sowing treatments. Seed treatments were sulphuric acid (95%), hot water (100°C), water with smoke paper disk, and demineralised water (control). The study revealed that all the treatments had a significant effect on germination with the exception of eight year old seeds obtained from C. subternata (seed source two). Although hot water treatment improved germination compared to smoked paper disk and the control, seeds treated with hot water degenerated rapidly. The highest overall germination (77.33%) was found with one year old seeds compared to other seed sources older than one year. Although smoked paper disks generally did not improve germination compared to the control, in one year old seeds from seed source one, this treatment greatly influenced germination, suggesting that seed age might have influenced germination of these seeds. In terms of germination rate, germination generally started after four days in most treatments.
- ItemThe evaluation and management of different grasses and legumes as potential cover crops in the vineyards of South Africa(Stellenbosch : University of Stellenbosch, 2007-03) Fourie, J.C.; Agenbag, G. A.; University of Stellenbosch. Faculty of Agrisciences. Dept. of Agronomy.A selection of species suitable for cover crop management in the different wine grape regions is required to enable more producers to apply this environment friendly practice in a sustainable manner as part of an integrated production strategy. The correct management practice(s) to be applied to these species over both the short and long term in a cooler and warmer wine grape region needed clarification. The effect of seeding date on the dry matter production (DMP) and weed control efficacy of seven grasses and sixteen legumes, as well as varieties of three of these species, was determined during 1991 and 1992. The decomposition rate of the mulches was determined. In the cooler climate of Stellenbosch (33o55'S, 18o52'E), the Medicago species, subterranean clovers, pink Seradella and three Vicia species did not compete effectively with the winter weeds if the weekly precipitation from mid-March to mid-May (autumn) exceeded 18 mm. The two oat species, as well as rye and triticale produced more than five t/ha of dry matter if the precipitation exceeded 18 mm per week. The DMP of the above-mentioned species indicated that these species could be considered for cover crop management in Lutzville (31o35'S, 18o52'E), if full surface irrigation of 18 mm per week could be applied for 10 weeks directly after sowing, followed by 18 mm fortnightly. Seeding date had a significant effect on DMP in both regions. A highly significant correlation (r = 0.85, p ≤ 0.0001) existed between the decomposition rate of the mulches and the initial amount of dry matter present on the soil surface.
- ItemNitrogen management strategies on perennial ryegrass-white clover pastures in the Western Cape Province(Stellenbosch : University of Stellenbosch, 2005-03) Labuschagne, Johan; Agenbag, G. A.; University of Stellenbosch. Faculty of Agrisciences. Dept. of Agronomy.The response of perennial ryegrass and white clover, grown under controlled conditions, to fertiliser N rates applied under variable soil temperature (6, 12 and 18 °C), soil water potential (-10, -20, -25 and -35 kPa) and seasonal growing (June/July and October/November) conditions as well as field conditions, were evaluated. Primary- (PDM), residual- (RDM) and total dry matter (TDM) production (g pot-1) were recorded over the first- and second regrowth cycles as well as the accumulative DM production over the two regrowth cycles, respectively. Leaf N content (%) was recorded at the end of first and second regrowth cycles. Tiller/stolon numbers and root dry mass (g pot-1) were recorded at the end of the second regrowth cycle. Soil ammonium-N and nitrate-N (mg kg-1) content was monitored after fertiliser N application. Decreasing soil temperatures resulted in decreased TDM production in both crops. Only perennial ryegrass was influenced by fertiliser N rate, with a general increase in dry matter production as fertiliser N rate was increased. Ryegrass TDM production did not differ between the 100 and 150 kg N ha-1 rates but were both higher (P=0.05) if compared to the 0 and 50 kg N ha-1 treatments. Soil nitrate levels 31 days after application of 150 kg N ha–1 were still sufficient to stimulate ryegrass RDM production. The 173.8% increase in ryegrass TDM production measured at 6 °C where 150 kg N ha-1 was applied compared to the 0 kg N ha-1 treatment illustrated the ability of ryegrass to respond to fertiliser N at low soil temperatures. Soil water potential of -20 kPa resulted in higher ryegrass PDM and TDM production compared to the -25 and -35 kPa levels. White clover PDM and TDM production were however not influenced by soil water potential or fertiliser N rate. Ryegrass TDM production increased (P=0.05) as fertiliser N rates were increased. The most favourable soil water level for both ryegrass and clover root development was found to be -35 kPa. Perennial ryegrass and white clover PDM, RDM and TDM production were higher during the October/November season compared to the June/July season. Increased fertiliser N rates resulted in increased (P=0.05) ryegrass PDM and TDM production. White clover dry matter production was not influenced by fertiliser N rates. In the field study the effect of 0, 50, 100 and 150 kg N ha-1 applied as a single application either in autumn, early winter, late winter, early spring or late spring on pasture dry matter production, clover content and selected quality parameters of a perennial ryegrass-white clover pasture were investigated. Soil nitrogen dynamics in the 0-100, 200-300 and 400-500 mm soil layers were studied for 49 days following fertiliser N application. The effect of 50 kg N ha-1 on soil N dynamics was generally the same as found at the 0 kg N ha-1 applications and may therefore be regarded as a low risk treatment. The application of 150 kg N ha-1 especially in autumn and early winter showed a tendency to exceed the absorption capacity of the pasture and thereby expose fertiliser N to possible leaching and contamination of natural resources. Increased fertiliser N rate resulted in a general increase in pasture dry matter production with the highest yields recorded where N was applied in early and late spring and the lowest in early winter. The application of 150 kg N ha-1 in early and late spring resulted in the highest TDM production, however, the 50 kg N ha-1 resulted in a more efficient conversion of N applied to additional DM produced. In contrast to DM production, the clover percentage generally decreased as fertiliser N rate was increased. The effect of season of application was inconsistent. Annual trends show that the clover percentage eventually recovered to the same levels as the 0 kg N ha-1 treatments. Due to the above minimum levels recorded for most mineral and quality parameters tested it is envisaged that treatment combinations as used in this study will not be at any disadvantage to pasture and animal productivity. The study has shown that the use of fertiliser N to boost perennial ryegrass-white clover productivity and thereby minimising the negative effect of the winter gap on fodder flow management during the cool season in the Western Cape Province, may be an important management tool. Except for late spring applications, all seasons of application reduced the negative impact of the winter gap on fodder availability. It is concluded that regression lines as summarised in Tables 7.2 and 8.2 show great potential to be instrumental in developing regression models, accurately predicting the effect of fertiliser N rate on pasture performance. Other factors to be considered includes the productivity of the pasture, initial clover content, expected clover content at the end of the first regrowth cycle after fertiliser N application and the quantity of additional fodder required. Additional requirements will be to maintain and 150 kg N ha-1) in winter, as the N uptake capacity of the pasture could be exceeded and thereby increasing the risk of N leaching, resulting in environmental pollution. The N response efficiency of the pasture is also the lowest at the 150 kg N ha-1 rates, thereby reducing the profitability of these treatments.
- ItemRangeland potential, quality and restoration strategies in North-Eastern Ethiopia : a case study conducted in the Southern Afar region(Stellenbosch : University of Stellenbosch, 2006-03) Gebremeskel, Kidane; Pieterse, P. J.; University of Stellenbosch. Faculty of Agrisciences. Dept. of Agronomy.Vegetation dynamics and restoration strategies of degraded rangeland were investigated near a watering point in the Allaidege communal grazing area in Administrative Zone 3 of the Afar Region in the northeastern lowlands of Ethiopia. The degradation gradient formed by grazing pressure in the study area was stratified into four different areas based on the vegetation cover; severely degraded (SD), moderately to severely degraded (MSD), moderately degraded (MD) and lightly degraded (LD) areas. The study was initiated at the start of the rainy season in June 2003 and lasted untill December 2004. The objectives were to study the effects of the grazing pressure on plant species composition; on plant biomass production and basal cover; on rangeland forage quality; on the rangeland soil status and to determine and quantify viable restoration strategies for forage species in severely degraded rangelands. The botanical composition of the different degradation areas was determined by making a 250 point wheel point method survey in each of four 30 m x 30 m quadrats in each degradation area using the nearest plant approach. The botanical composition of each degradation area was determined by measuring the frequency of occurrence of the different life forms (perennial grasses, annual grasses and forbs) of the species recorded in the field. Accordingly, a significant interaction was observed in both seasons between the different degradation areas and life forms considered. A high abundance of annual grasses was evident in SD and MSD areas in both seasons. In the MD and LD areas, a three-fold increase in frequency was recorded for perennial grasses compared to the MSD area in 2003. In 2004, the frequency of annual grasses, forbs and perennial grasses in the MD area was almost similar to that of the LD area. The abundance of perennial grasses in the MD and LD areas was two- and five-fold higher compared to perennial grasses in the MSD and SD areas respectively. Biomass production was recorded by cutting the vegetation in 1 m x 1 m quadrats in each grazing area at ground level. The dry matter content of subsamples was determined in order to calculate the dry matter production of the quadrat. The differences in dry matter yield recorded in the different degraded areas was not significant for the 2003 season, although an increasing trend in yield was observed from the SD to MD areas. Significant yield differences were however recorded when one outlier in the data was excluded from the analysis. The significant differences occurred between the MD and SD areas where the MD area produced 2.4 t ha-1 more dry matter than the SD area. Similarly, in 2004 no significant yield difference was observed between the degradation areas. However, the contribution of different species to dry matter yield varied in the different degradation areas. Setaria verticillata, Sporobolus ioclados and Paspalidium desertorum were found to be the major species contributing to the dry matter producion in the SD area, S. verticillata and P. desertorum in the MSD area, Chrysopogon plumulosus and P. desertorum in the MD area and C. plumulosus and Panicum coloratum in the LD area. The percentage basal cover was calculated from the number of basal strikes recorded at 1 000 points in each plot of each degradation area using the wheel point method. The total basal cover percentage did not significantly change along the degradation gradient in any of the seasons. However, data for both seasons showed an increasing trend of total basal cover percentage closer to the watering point compared to areas further away from the watering point, except for the SD area, which had the lowest basal cover percentage. The contribution to percentage basal cover by some species decreased while it increased for some other species in grazing areas near the watering point. Forage quality was investigated by analysing sub-samples of the forage samples taken to determine biomass production. The forage samples were analysed for neutral detergent fibre (NDF), acid detergent fibre (ADF), crude protein (CP), lignin, in vitro dry matter digestibility (IVDMD), phosphorus (P), and calcium (Ca) content. The forage showed a decrease in NDF and ADF content in areas close to the watering point in both seasons. This decrease in fibre content was accompanied by an increase in CP content close to the watering point. The increase in CP was significant for the SD area in both seasons. Although a similar trend was observed in both seasons, the CP content was found to be significantly higher in 2004 than in 2003. The results of the lignin analysis were inconclusive if the data of both seasons are considered. It does appear however as if the lignin content of the forage was generally higher in 2003. The 2 years pooled average of P content of the forages showed insignificant variation along the degradation gradient. However, an increase in P concentration of the forages was evident in areas far from the watering point. Contrary to this, Ca concentration was significantly higher in the SD area compared to areas further away from the watering point. Hand clipped forage samples and esophageal collected forage samples were analysed to compare the quality of the samples. Due to the fact that only two animals were available for esophageal collection, differences were in most cases not significant at the 5% level, but trends indicate that animals select higher quality forage than what is assumed based on hand clipping. Organic carbon (OC) content, total nitrogen (N) content, available phosphorus (P) content, available potassium (K) content, exchangeable calcium (Ca) and magnesium (Mg) contents, cation exchange capacity (CEC), total exchangeable bases (TEB), exchangeable sodium percentage (ESP), soil acidity (pH) and base saturation of soils in the different degradation areas were determined by means of acknowledged laboratory methods. No significant differences in OC, N, P, K, Ca, Mg and K content of soil in the different degradation areas could be observed. There was however an increasing trend for OC and N content with distance from the watering point. Sodium concentration and pH increased significantly in areas close to the watering point. Cation exchange capacity content of the soil was variable and no clear trend could be established. Significantly higher TEB and ESP contents were observed in the SD area. In general, the differences in plant biomass production and basal cover, botanical composition, forage quality and soil status over the degradation gradient clearly implicates the negative impact of unrestricted grazing pressure on the rangeland around the watering points. In the rangeland restoration trial, establishment of three local and three exotic grass species in the SD area was investigated. Treatments applied included application of inorganic fertilizer, dry dung organic manure and grass mulch. The mulch treatments caused a significant yield increase for all the sown species. Among all the species, Ischaemum afrum and Tragus berteronianus performed better and produced significantly higher dry matter yields than Enteropogon rupestris, Chloris gayana and Panicum coloratum. In general the study indicated the importance of mulching when planning to restore degraded rangeland under arid environmental conditions.