Masters Degrees (Clinical Pharmacology)

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Browsing Masters Degrees (Clinical Pharmacology) by Subject "BCG vaccines"

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    Karakterisering van die katalase van BCG : invloed van isoniasied op die gesuiwerde ensiem
    (Stellenbosch : Stellenbosch University, 1990-12) Basson, Karen; Van Zyl, J. M.; Van der Walt, B. J.; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Clinical Pharmacology.
    ENGLISH ABSTRACT: Catalase was extracted and isolated from BCG bacilli by the following precedures : sonication, treatment with deoxyribonuclease, treatment with octyl glucoside and sonification, ammonium sulphate precipitation, hydroxyapatite chromatography and chromatography on Sephacryl S-1000. A significant increase in the specific activity of the catalase and peroxidase functions was observed during purification and a final purity index (A405/A280) of 0.258 was obtained. Only one protein band, in addition to aggregates which could hardly penetrate the gel, was observed after non-denaturing polyacrylamide gel electrophoresis of the purified enzyme. The peroxidase activity (ABTS) assay) was consistent with the protein band. A.M. of 163.1 kDa was calculated from results obtained with SDS polyacrylamide gel electrophoresis, which correlates with a Mr of 144.54 kDa obtained by gelfiltration studies on Sephacryl S-1000. A characteristic Soret peak at 405 nm was observed on the UV spectrum of the purified enzyme. The optimum pH for catalase activity was 8,0. At pH 7,0 81% of the maximum activity at pH 6,0 and 56% at pH 9,0. This correlates with the fact that catalase must be able to survive over a broad pH range.