Masters Degrees (Biochemistry)

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The effect of adenylate kinase on the glycolytic oscillations of Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2019-03) Van Wyk, Morne; Snoep, Jacob Leendert; Van Niekerk, David; Kouril, Jutta Theresa; Stellenbosch University. Faculty of Science. Dept. of Biochemistry.
ENGLISH ABSTRACT: The regulation of phosphofructokinase (PFK) by ATP and AMP is a necessary feature for glycolytic oscillations. The enzyme adenylate kinase (AK) is responsible for the interconversion of ATP + AMP 2 ADP, and so influences the regulation of PFK. We present here, both experimentally and through model simulations, that a decrease in AK activity results in an increase in period time of the glycolytic oscillations. We show, for the rst time, that AK is a necessary component of these oscillations and an absence of AK activity results in no oscillations. Using an existing model for glycolytic oscillations, we incorperate a mechanistic equation for AK activity with parameters determined from ts to enzyme kinetic data. In the subsequent model simulations we piece apart the individual contributions of ATP and AMP towards these oscillations and find that a reduction in AK activity leads to changes in both ATP and AMP oscillation phase and amplitude. This causes changes in PFK T and R state switching, increasing the period time of the oscillations.