Browsing by Author "Van Vuuren, H. J. J."

Now showing 1 - 6 of 6
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    Characterization of killer yeast isolates from chenin blanc grapes and grape skins
    (South African Society for Enology and Viticulture, 1991) Jacobs, C. J.; Fourie, I.; Van Vuuren, H. J. J.
    Wild-type killer yeast strains isolated from six South African wineries were identified using classical taxonomic methods. They were further characterized according to their cross-reactions with reference killer yeasts (K1-Kn) and by electrophoresis of their double-stranded RNA molecules. All isolates belonged to the K2 phenotype and were identified as strains of Saccharomyces cerevisiae and Saccharomyces bayanus. The killer strains differed substantially in their ability to kill a sensitive wine yeast (Geisenheim GS-1). This phenomenon may be attributed to strain differences among the killer yeasts as was shown by electrophoresis of total soluble cell proteins and gas chromatographic analysis of cellular fatty acids.
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    Killer yeasts - cause of stuck fermentations in a wine cellar
    (South African Society for Enology and Viticulture, 1986) Van Vuuren, H. J. J.; Wingfield, Brenda D.
    Sluggish fermentations in five fermenters in a wine cellar were investigated. Methylene blue-stains of yeast suspensions revealed that approximately 90% of the total yeast population in each of the fermenters were dead. The viable cells in each fermenter were killer yeasts. Polyacrylamide gel electrophoresis of total soluble cell proteins showed that the same killer yeast occurred in each of the five fermenters. The effect of killer yeast on viability and fermentation activity of the wine yeast was studied in an enriched grape juice medium at 20°C and 30°C. Death rate of the wine yeast was considerably higher in the presence of the killer yeast and fermentations were retarded at both temperatures. The killer yeast induced flocculation of the non-flocculent wine yeast.
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    Malic acid distribution and degradation in grape must during skin contact : the influence of recombinant malo-ethanolic wine yeast strains
    (South African Society for Enology and Viticulture, 2005) Van Staden, J.; Volschenk, H.; Van Vuuren, H. J. J.; Viljoen-Bloom, M.
    Wine acidity plays an important role in determining wine quality and ensuring physiochemical and microbiological stability. In high-acid wines, the L-malic acid concentration is usually reduced through bacterial malolactic fermentation, while acidulation in low-acidity wines is usually done during final blending of the wine before bottling. This study showed that skin contact did not influence the relative concentration of L-malic acid in the pulp and juice fractions from Colombard, Ruby Cabernet and Cabernet Sauvignon grape musts, with 32%-44% of the L-malic acid present in the pulp fraction. Four recombinant malo-ethanolic (ME) Saccharomyces wine yeast strains containing the malic enzyme (mae2) and malate transporter (mael) genes of Schizasaccharomyces pombe, effectively degraded the L-malic acid in both the juice and pulp fractions of all three cultivars, with a complete degradation of malic acid in the juice fraction within 2 days.
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    Malic acid in wine : origin, function and metabolism during vinification
    (South African Society for Enology and Viticulture, 2006) Volschenk, H.; Van Vuuren, H. J. J.; Viljoen-Bloom, M.
    The production of quality wines requires a judicious balance between the sugar, acid and flavour components of wine. L-Malic and tartaric acids are the most prominent organic acids in wine and play a crucial role in the winemaking process, including the organoleptic quality and the physical, biochemical and microbial stability of wine. Deacidification of grape must and wine is often required for the production of well-balanced wines. Malolactic fermentation induced by the addition of malolactic starter cultures, regarded as the preferred method for naturally reducing wine acidity, efficiently decreases the acidic taste of wine, improves the microbial stability and modifies to some extent the organoleptic character of wine. However, the recurrent phenomenon of delayed or sluggish malolactic fermentation often causes interruption of cellar operations, while the malolactic fermentation is not always compatible with certain styles of wine. Commercial wine yeast strains of Saccharomyces are generally unable to degrade L-malic acid effectively in grape must during alcoholic fermentation, with relatively minor modifications in total acidity during vinification. Functional expression of the malolactic pathway genes, i.e. the malate transporter (mae1) of Schizosaccharomyces pombe and the malolactic enzyme (mleA) from Oenococcus oeni in wine yeasts, has paved the way for the construction of malate-degrading strains of Saccharomyces for commercial winemaking.
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    Relationship between ergosterol concentrations in wine yeast and sugar fermentation at different temperatures
    (South African Society for Enology and Viticulture, 1989) Venter, J. J.; Van Vuuren, H. J. J.; Tromp, A.; Randall, J. H.
    The effect of cellular ergosterol on the fermentation ability of four different Saccharomyces cerevisiae wine yeast strains was studied. F~rmentations were carried out at three temperatures and ergosterol concentrations determined at six fermentation stages. The lower ergosterol concentrations at the higher temperatures are ascribed to the higher growth rate of the yeast cells resulting in a more rapid dilution of the ergosterol. Growth of yeast during fermentation was linked with the ergosterol content of the cells. The ergosterol concentration of the yeast cells was not directly correlated to specific fermentation rates.
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    Relationship between fatty acid concentrations in wine yeasts and sugar fermentation at different temperatures
    (South African Society for Enology and Viticulture, 1989) Venter, J. J.; Van Vuuren, H. J. J.; Tromp, A.; Randall, J. H.
    The effects of temperature and fermentation stage on the cellular concentrations of unsaturated and saturated fatty acids on the fermentation abilities of four different Saccharomyces cerevisiae wine yeasts were studied at three temperatures. The total unsaturated and saturated fatty acid concentrations were determined at six fermentation stages. Unsaturated fatty acid concentration decreased and the concentration of saturated fatty acids increased as fermentation proceeded. Saturated fatty acid concentration of the yeast cells correlated positively with high specific fermentation rates during the later stages of fermentation in contrast to the belief that fluid membranes with high unsaturated fatty acid concentrations enhance ethanol tolerance and, therefore, fermentation performance.