SUNScholar - Browsing by Author "Stander, F. S. H."

Browsing by Author "Stander, F. S. H."

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A comparative analysis of grade 1 and grade 2 water in the Tygerberg Hospital in vitro fertilisation programme
(Health and Medical Publishing Group (HMPG), 1987-02) Kruger, T. F.; Van Wyk, H.; Stander, F. S. H.; Smith, K.; Menkveld, R.; Van der Merwe, J. P.
There are conflicting reports in the literature about the effect of the water source for growth and insemination media in an in vitro fertilisation (IVF) programme. A controlled trial was carried out in which two-cell mouse embryos were cultured in Ham F10 medium containing either grade 1 water (test group) or grade 2 water (control group). Of the two-cell embryos, 92,0% (69 of 75) in the test group and 91,8% (67 of 73) in the control group cleaved to the blastocyst stage (no statistically significant difference). On the basis of this experiment grade 2 water was used in the Tygerberg Hospital human IVF programme with good results. A continuing pregnancy rate of 23% per embryo transfer over a 1-year period is reported.
The development of one- and two-cell mouse embryos in the absence of human serum
(Health & Medical Publishing Group, 1986) Kruger, T. F.; Stander, F. S. H.; Smith, K.; Lombard, C. J.
One- and two-cell embryos were obtained from F1 hybrid female mice stimulated with human menopausal gonadotrophin and randomly distributed into two groups - group 1 (no serum) and group 2 (10% patient's serum). Fifty of 53 (94.3%) one-cell embryos in group 1 had cleaved to the blastocyst stage and 44 of 49 (89.79%) in group 2 after 96 hours (no significant difference - chi-square test). In the two-cell embryos in group 1, 78 of 89 (87.6%) reached the blastocyst stage after 72 hours and 80 of 86 (93.02%) in group 2 (no significant difference - chi-square test). According to microscopic evaluation cleavage to the blastocyst stage without serum supplementation is possible. The value of serum is discussed.
The effect of fluorescent light on the cleavage of two-cell mouse embryos
(Health & Medical Publishing Group, 1985) Kruger, T. F.; Stander, F. S. H.
Two-cell mouse embryos were subjected to fluorescent light, 2,900 lux, for 30 minutes, and the cleavage compared with that in a control group. There was no statistically significant difference in the results. In both groups 90% of two-cell embroys reached the expected level of cleavage. The possible effect of fluorescent light on the oocyte is discussed.
The effect of surgical glove powder on cleavage of two-cell mouse embryos in an in vitro fertilization programme
(Health & Medical Publishing Group, 1985) Kruger, T. F.; Cronje, H. S.; Stander, F. S. H.; Menkveld, R.; Conradie, E.
The effect of surgical glove powder on the development of early mouse embryos was studied. Embryos from F1 hybrid mice (C57 B1/6 x CBA) were suspended in Whittingham's T6 growth medium with 10% human serum, using Petri dishes (Falcon 3001). Contamination was brought about by a sterile, powdered, surgical glove touching the surface of the growth medium for less than a second in group I, and in group II the same procedure was followed but the glove was rinsed beforehand with sterile, four times distilled water and air-dried. In the control group (group III) no contamination with surgical glove powder occurred. In group I only 9 of 137 embryos (7%) reached the blastocyst stage, in contrast with 110 of 196 (56%) in group II and 258 of 287 (90%) in group III. The differences in results between groups I and III, groups I and II, and groups II and III were found to be statistically significant (P < 0.001) by the chi-square test. It is concluded that surgical gloves are a potent inhibitor of early embryonic growth. In an in vitro fertilization programme including follicle aspiration and embryo transfer, contamination of embryos with these gloves should be avoided at all costs.
The effect on cleavage of two-cell mouse embryos after a delay in embryo retrieval in a human in vitro fertilization programme
(Health & Medical Publishing Group, 1985) Kruger, T. F.; Stander, F. S. H.
Two-cell mouse embryos are used for quality control in a human in vitro fertilization programme. A controlled experiment was designed to evaluate the effect on cleavage of two-cell mouse embryos after a delay in embryo retrieval. In the test group, two fallopian tubes were incubated in Whittingham's T6 medium for 2 1/2 hours per experiment before the embryos were removed for culture. In the control group embryos were removed from the fallopian tubes immediately after the mice were sacrificed. Five experiments were performed. Eight of 141 two-cell embryos (5,7%) reached the balstocyst stage in the test group, and in the control group 143 of 151 two-cell embryos (94,7%) reached the blastocyst stage after 72 hours. Embryos must be removed immediately after the mice are sacrificed to obtain constant results. If not, poor cleavage can lead to unnecessary confusion in the laboratory.
Factors affecting pregnancy outcome in a gamete intrafallopian transfer (GIF) programme
(Health & Medical Publishing Group, 2003-07) De Bruijn, J. M.; Kruger, T. F.; Van der Merwe, J. P.; Stander, F. S. H.; Lombard, C. J.
Objective. To identify the factors that most significantly affected pregnancy rates in a gamete intrafallopian transfer (GIFT) programme. Methods. A total of 863 GIFT cycles were analysed retrospectively. The variables found to be associated significantly with pregnancy were then used to obtain multivariate analysis using logistical regression. Results. Overall and ongoing pregnancy rates were significantly better in patients ≤ 38 years than in patients > 38 years (37.3% and 28.4% v. 23.7% and 11.0% respectively), and age was positively associated with success after GIFT (odds ratio (OR) 1.87, 95% confidence interval (CI): 1.22 - 2.85). Metaphase I (MI) oocytes were negatively associated with pregnancy (OR 1.54, 95% CI: 0.28 - 1.04). The highest pregnancy rates occurred when 3 metaphase II (MII) oocytes were transferred (39.8%, OR 7.51, 95% CI: 1.74 - 32.42). With regard to sperm morphology, overall pregnancy rates of 25.5% (≤ 4% normal forms) and 37.2% (> 4% normal forms) were obtained. Morphology of > 4% normal forms was positively associated with pregnancy (OR 1.58, 95% CI: 1.04 - 2.42). Conclusion. The results of this study suggest that the most important factors influencing pregnancy rates in a GIFT programme are the woman's age and those factors pertaining to the characteristics of the gametes. Considering the emotional and financial costs it is important to relate this information to all prospective participants in a GIFT programme.
Human pregnancy after transfer of intact frozen-thawed embryos
(Health & Medical Publishing Group, 1989) Erasmus, E.L.; Van der Merwe, J. P.; Kruger, T. F.; Stander, F. S. H.; Menkveld, R.
Since the birth of the first baby as a result of in vitro fertilisation (IVF) in 1978, many clinics around the world have achieved pregnancies and births for their patients by using IVF and gamete intrafallopian transfer procedure. With the storage of excess embryos, multiple laparoscopies can be avoided; this has favoured in the development of better cryopreservation techniques. In our clinic 8-cell human embryos are frozen in a 1,5M dimethyl sulphoxide solution as cryoprotectant using the slow freeze-thaw method. Sixteen thawed embryos were replaced in 8 patients, resulting in 1 pregnancy. Of the thawed embryos 51,6% survived the freezing process in that they had 50% or more of the original number of blastomeres and also the zona pellucida intact.
The in vitro fertilisation programme at Tygerberg Hospital and the University of Stellenbosch. Five years' experience, April 1983 - January 1988
(Health & Medical Publishing Group, 1990) Kruger, T. F.; Van der Merwe, J. P.; Odendaal, H. J.; Stander, F. S. H.; Grobler, G. M.; Hulme, V. A.; Erasmus, E. L.; Coetzee, K.; Windt, M.-L.; Swart, Y.; Smith, K.; Menkveld, R.
The results of the in vitro fertilisation programme at Tygerberg Hospital for the period April 1983 to January 1988 are presented. Of the 1117 laparoscopies performed, 825 patients reached the transfer stage. A live-birth rate of 9.3% was achieved. The pregnancy rate after transfer of 4 embryos was 25,9% compared with 15,4% after 2 embryos and 10,8% after 3 embryos (P = 0.0001). The multiple pregnancy rate was 2.8% in the group receiving 2 embryos and 11.7% and 10,4% in those receiving 3 and 4 embryos, respectively. Of the 77 successful pregnancies (90 babies), 1 baby died at 34 weeks' gestation as the result of abruptio placentae due to preeclampsia and 1 cot death occurred. The only congenital abnormality encountered was a cleft palate.
Osmolarity studies with different containers and volumes in a human in vitro fertilization programme
(Health and Medical Publishing Group (HMPG), 1985-10) Kruger, T. F.; Stander, F. S. H.; Menkveld, R.; Lombard, C. J.
[No abstract available]
Results of phase I of the in vitro fertilization and embryo transfer programme at Tygerberg Hospital
(Health and Medical Publishing Group (HMPG), 1985-05) Kruger, T. F.; Van Schouwenburg, J. A. M. H.; Stander, F. S. H.; Van den Heever, A. D.; Van Zyl, J. A.; Menkveld, R.; Kopper, K.; De Villiers, A.; Conradie, E.; Odendaal, H. J.; De Villiers, J. N.
Phase I (3 June - 31 October 1983) of the in vitro fertilization (IVF) programme at Tygerberg Hospital is outlined in this article. This programme led to the birth of the first IVF baby in South Africa after fertilization and embryo transfer (ET) took place at this institution. The baby was born on 29 April 1984; a second baby was born on 20 June 1984. During phase I 24 laparoscopies were performed; 32 oocytes were obtained from 56 follicles, of which 62.5% were fertilized. Eleven ETs were performed, which led to 3 pregnancies. An ongoing pregnancy rate of 18% per ET and a pregnancy rate of 12,5% per laparoscopy were achieved.
Results of the in vitro fertilization programme at Tygerberg Hospital, phases II and III
(Health & Medical Publishing Group, 1986-03) Kruger, T. F.; Van der Merwe, J. P.; Stander, F. S. H.; Menkveld, R.; Van den Heever, A. D.; Kopper, K.; Odendaal, H. J.; Van Zyl, J. A.; De Villiers, J. N.
Phases II and III of the human in vitro fertilization programme at Tygerberg Hospital are presented. In phase II, 42 laparoscopies were performed and oocytes were obtained from 76% of the follicles aspirated, but with a fertilization rate of only 37%. The viable pregnancy rate per embryo transfer was 4%. Important changes took place in the programme, which led to a fertilization rate of 77% in phase III. Of the 78 patients subjected to laparoscopy, 65 (83%) reached the embryo transfer stage, resulting in a clinical pregnancy rate per embryo transfer of 23%, and with a 19% pregnancy rate per laparoscopy. The changes, methods and results of phases II and III are discussed.
'n Vergelykende studie tussen tweeselembrio's van CBA- en F1-muise in 'n menslike in vitro bevrugtingsprogram
(Health and Medical Publishing Group (HMPG), 1984-02) Kruger, T. F.; Stander, F. S. H.
The two-cell mouse embryo can develop in a chemically defined medium to the blastocyst stage but conditions must be strictly controlled. Fresh T6 (Whittingham's) medium is being used weekly for this purpose in our laboratory and subjected to a 5% CO2-in-air system 24 hours prior to use. Experiments were designed to determine whether two-cell embryos obtained from an F1 (CBA x C57/B1/6) x F1 cross were superior to the easily obtainable CBA x ICR cross. The aim was to get a 90% blastocyst result for use as a quality control. Eight experiments were performed over a 6-week period. The CBA two-cell embryos were compared with the F1 group under identical conditions. A total of 177 two-cell embryos were obtained from the CBA group and 214 two-cell embryos from the F1 group. In the CBA group 51 developed to the blastocyst stage (28.8%) compared with 187 in the F1 group (87,4%) (P<0.0001). In conclusion, it is advisable to obtain F1 mice to use weekly in the laboratory as a quality control measure. The CBA group yields poor cultures, and this lead to unnecessary laboratory confusion.
'n Vergelykende studie van die witselpipet- en Makler-telkamermetodes vir die tel van spermatosoa
(Health and Medical Publishing Group (HMPG), 1980-10) Menkveld, R.; Van Zyl, J. A.; Stander, F. S. H.; Conradie, E.; Kopper, K.
A new method for counting spermatozoa was introduced by Makler. This method was compared with the white blood cell pipette method and the results revealed high precision and accuracy (difference of means = 5,9%). Makler's method, which is very simple to perform, has the advantage that rapid and reliable sperm counts can be done. Therefore, the Makler counting chamber can be used with confidence, in the performance of semen analysis by any doctor or in any laboratory.

Browsing by Author "Stander, F. S. H."

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