Browsing by Author "Mphahlele, Rebogile R."

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    Drying kinetics of pomegranate fruit peel (cv. Wonderful)
    (Elsevier, 2019) Mphahlele, Rebogile R.; Pathare, Pankaj B.; Opara, Umezuruike Linus
    Pomegranate juice processing produces large amount of peel as by-product or waste which is a potential source of raw material for food and other bioprocessing industries. Drying the peel offers opportunities for value addition into novel products, thus reducing waste from the fruit processing operations. This study presents the mathematical models of thin layer drying behaviour of pomegranate peels (initial thickness 5.00 ± 0.05 mm and moisture content 70.30% wet basis) using three air temperatures (40 °C, 50 °C and 60 °C) at a constant air velocity of 1.0 m/s. The results obtained showed that drying time decreased as the oven drying temperature increased. The drying process took place mainly in the falling rate period. Ten thin layer drying models were evaluated based on coefficient of determination (r2) and standard error (es). Among the tested drying models, Midilli et al. mathematical model was found to be the best fit for establishing the drying kinetics of pomegranate peel. Furthermore, the effective moisture diffusivity of pomegranate peel ranged from 4.05 × 10−10 to 8.10 × 10−10 m2/s over the temperature range investigated, with mean activation energy (Ea) of 22.25 kJ/mol.
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    Effect of drying on the bioactive compounds, antioxidant, antibacterial and antityrosinase activities of pomegranate peel
    (BioMed Central, 2016) Mphahlele, Rebogile R.; Fawole, Olaniyi A.; Makunga, Nokwanda P.; Opara, Umezuruike L.
    The use of pomegranate peel is highly associated with its rich phenolic concentration. Series of drying methods are recommended since bioactive compounds are highly sensitive to thermal degradation. The study was conducted to evaluate the effects of drying on the bioactive compounds, antioxidant as well as antibacterial and antityrosinase activities of pomegranate peel. Methods Dried pomegranate peels with the initial moisture content of 70.30 % wet basis were prepared by freeze and oven drying at 40, 50 and 60 °C. Difference in CIE-LAB, chroma (C*) and hue angle (h°) were determined using colorimeter. Individual polyphenol retention was determined using LC-MS and LC-MSE while total phenolics concentration (TPC), total flavonoid concentration (TFC), total tannins concentration (TTC) and vitamin C concentration were measured using colorimetric methods. The antioxidant activity was measured by radical scavenging activity (RSA) and ferric reducing antioxidant power (FRAP). Furthermore, the antibacterial activity of methanolic peel extracts were tested on Gram negative (Escherichia coli and Klebsiella pneumonia) and Gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro microdilution assays. Tyrosinase enzyme inhibition was investigated against monophenolase (tyrosine) and diphenolase (DOPA), with arbutin as positive controls. Results Oven drying at 60 °C resulted in high punicalin concentration (888.04 ± 141.03 mg CE/kg dried matter) along with poor red coloration (high hue angle). Freeze dried peel contained higher catechin concentration (674.51 mg/kg drying matter) + catechin and –epicatechin (70.56 mg/kg drying matter) compared to oven dried peel. Furthermore, freeze dried peel had the highest total phenolic, tannin and flavonoid concentrations compared to oven dried peel over the temperature range studied. High concentration of vitamin C (31.19 μg AAE/g dried matter) was observed in the oven dried (40 °C) pomegranate peel. Drying at 50 °C showed the highest inhibitory activity with the MIC values of 0.10 mg/ml against Gram positive (Staphylococcus aureus and Bacillus subtili. Likewise, the extracts dried at 50 °C showed potent inhibitory activity concentration (22.95 mg/ml) against monophenolase. Principal component analysis showed that the peel colour characteristics and bioactive compounds isolated the investigated drying method. Conclusions The freeze and oven dried peel extracts exhibited a significant antibacterial and antioxidant activities. The freeze drying method had higher total phenolic, tannin and flavonoid concentration therefore can be explored as a feasible method for processing pomegranate peel to ensure retention of the maximum amount of their naturally occurring bioactive compounds.