Browsing by Author "Hussain, Hidayat"

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    4-Benzyloxylonchocarpin and muracatanes A-C from Ranunculus muricatus L. and their biological effects
    (MDPI, 2020-11-17) Hussain, Hidayat; Ali, Iftikhar; Wang, Daijie; Mamadalieva, Nilufar Z.; Hussain, Wahid; Csuk, Rene; Loesche, Anne; Fischer, Lucie; Staerk, Dan; Anam, Syariful; AlZain, Mashail N.; Mushtaq, Maria; Ul-Haq, Zaheer; Ullah, Riaz; Noman, Omar M.; Abbas, Ghulam; Green, Ivan R.
    ENGLISH ABSTRACT: Ranunculus muricatus L. is a spiny fruit buttercup that is used in various traditional medicinal systems. In the current investigation of R. muricatus, the new chalcone 4-benzyloxylonchocarpin (1), the new anthraquinone muracatanes A (2), the new-to-nature anthraquinone muracatanes B (3), and the new naphthalene analog muracatanes C (4) were isolated, in addition to the three previously reported compounds, 4-methoxylonchocarpin (5), β-sitosterol (6), and β-sitosterol β-D-glucopyranoside (7). Their structures were elucidated using 1D (1H and 13C) and 2D (COSY, HSQC, and HMBC) NMR spectroscopy and HR-ESI-MS. Chalcone 1 showed potent acetylcholinesterase inhibitory effects with Ki of 5.39 µM and Ki′ of 3.54 µM, but none of the isolated compounds showed inhibitory activity towards butyrylcholinesterase. Anthraquinone 3 illustrated α-glucosidase inhibitory effects with IC50-values of 164.46 ± 83.04 µM. Compound 5 displayed moderate cytotoxic activity towards ovarian carcinoma (A2780, IC50 = 25.4 µM), colorectal adenocarcinoma (HT29, IC50 = 20.2 µM), breast cancer (MCF7, IC50 = 23.7 µM), and thyroid carcinoma (SW1736, IC50 = 26.2 µM) while it was inactive towards pharynx carcinoma (FaDu: IC50 > 30 µM).
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    Chemical constituents isolated from Lycium shawii and their chemotaxonomic significance
    (ACG Publications, 2017-11-15) Rehman, Najeeb Ur; Hussain, Hidayat; Al-Riyami, Samia Ahmed; Green, Ivan. R.; Al-Harrasi, Ahmed
    ENGLISH ABSTRACT: Phytochemical investigation of Lycium shawii Roem. & Schult provided fourteen compounds, including lyciumate (1), dehydrocostus lactone (2), costunolide (3), catechin (4), lyciumaside (5), emodin (6), emodin-8-O-β-D-glucoside (7), aloe-emodin (8), aloe-emodin-8-O-β-D-glucoside (9), aloe emodin-11-O-rhamnoside (10), chrysophanol-8-O-β-D-glucoside (11), nonacosane-10-ol (12), betulinic acid (13) and β-sitosterol glucopyranoside (14). The compounds may be classified as three sesquiterpene lactones (1-3), two phenolic compounds (4 and 5), six anthraquinones (6-11), one long chain alcohol (12), one lupane-type triterpenoid (13) and a steroid (14). Compounds 2, 3, 7 and 9-12 are reported here for the first time as isolated from any species of Lycium as well as from the Solanaceae family while compounds 8, 13 and 14 are reported to be found for the first time in the genus Lycium. All structural assignments were made by comparing the NMR spectral data of the pure isolates with that published in the quoted literature.