Browsing by Author "Badrodien, Imraan"

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    Light sheet fluorescence microscopy
    (Stellenbosch : Stellenbosch University, 2021-03) Badrodien, Imraan; Bosman, Gurthwin W.; Neethling, Pieter H.; Stellenbosch University. Faculty of Science. Dept. of Physics.
    ENGLISH ABSTRACT: Light sheet fluorescence microscopy is a powerful tool within the in field of microscopy. The inherent advantages over other fluorescence microscopy techniques include high sectioning capabilities, reduced photo-damage in the sample and short data acquisition times. In this thesis, a light sheet microscope is developed which allows the users to tailor the parameters of the light sheet for various applications, by implementing the use of a spatial light modulator (SLM) to dynamically alter the shape of the light sheet. Three different techniques for light sheet generation are investigated, namely by the use of a cylindrical lens, using an SLM, and by digitally scanning a beam. Each of the techniques were characterised. Using the light sheets, three dimensional fluorescence images are obtained. These three dimensional images are analysed to determine the imaging capabilities of the system, and the deconvolution of these images are implemented for image restoration. The result is a multi-purpose light sheet microscope for use in biological imaging.