Using µCT in live larvae of a large wood-boring beetle to study tracheal oxygen supply during development

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dc.contributor.authorLehmann, Philippen_ZA
dc.contributor.authorJaval, Marionen_ZA
dc.contributor.authorDu Plessis, Antonen_ZA
dc.contributor.authorTerblanche, John S.en_ZA
dc.date.accessioned2021-05-28T09:03:06Z
dc.date.accessioned2021-08-25T15:51:31Z
dc.date.available2021-05-28T09:03:06Z
dc.date.available2021-08-25T15:51:31Z
dc.date.issued2021
dc.descriptionCITATION: Lehmann, P. et al. 2021 Using µCT in live larvae of a large wood-boring beetle to study tracheal oxygen supply during development. Journal of Insect Physiology 130, 104199, doi10.1016/j.jinsphys.2021.104199.
dc.description.abstractENGLISH ABSTRACT: How respiratory structures vary with, or are constrained by, an animal's environment is of central importance to diverse evolutionary and comparative physiology hypotheses. To date, quantifying insect respiratory structures and their variation has remained challenging due to their microscopic size, hence only a handful of species have been examined. Several methods for imaging insect respiratory systems are available, in many cases however, the analytical process is lethal, destructive, time consuming and labour intensive. Here, we explore and test a different approach to measuring tracheal volume using X-ray micro-tomography (mu CT) scanning (at 15 mu m resolution) on living, sedated larvae of the cerambycid beetle Cacosceles newmannii across a range of body sizes at two points in development. We provide novel data on resistance of the larvae to the radiation dose absorbed during mu CT scanning, repeatability of imaging analyses both within and between time-points and, structural tracheal trait differences provided by different image segmentation methods. By comparing how tracheal dimension (reflecting metabolic supply) and basal metabolic rate (reflecting metabolic demand) increase with mass, we show that tracheal oxygen supply capacity increases during development at a comparable, or even higher rate than metabolic demand. Given that abundant gas delivery capacity in the insect respiratory system may be costly (due to e.g. oxygen toxicity or space restrictions), there are probably balancing factors requiring such a capacity that are not linked to direct tissue oxygen demand and that have not been thoroughly elucidated to date, including CO2 efflux. Our study provides methodological insights and novel biological data on key issues in rapidly quantifying insect respiratory anatomy on live insects.en_ZA
dc.description.versionPublisher's version
dc.format.extent9 pages : illustrationsen_ZA
dc.identifier.citationLehmann, P.; Javal, M.; Du Plessis, A.; Terblanche, J.S. (2021). Using µCT in live larvae of a large wood-boring beetle to study tracheal oxygen supply during development. Journal of Insect Physiology 130, 104199, 9 pages. DOI: 10.1016/j.jinsphys.2021.104199.
dc.identifier.issn1879-1611 (online)
dc.identifier.urihttp://hdl.handle.net/10019.1/113590
dc.publisherElsevier
dc.rights.holderAuthors retain copyright
dc.subject.lcshWood borers -- Ecophysiologyen_ZA
dc.subject.lcshInsects -- Respiratory organsen_ZA
dc.subject.lcshBeetles -- Morphologyen_ZA
dc.subject.lcshColeoptera -- Respirationen_ZA
dc.subject.lcshTrachea -- Developmenten_ZA
dc.subject.lcshMicrotomographyen_ZA
dc.subject.lcshBeetles -- Larvae -- Ecologyen_ZA
dc.subject.lcshBeetle rearingen_ZA
dc.titleUsing µCT in live larvae of a large wood-boring beetle to study tracheal oxygen supply during developmenten_ZA
dc.typeArticleen_ZA
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