Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae alpha-amylase in industrial strains of Saccharomyces cerevisiae

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dc.contributor.authorGundllapalli Moses, S. B.
dc.contributor.authorCordero Otero, R. R.
dc.contributor.authorLa Grange, Daniel C.
dc.contributor.authorVan Rensburg, Pierre
dc.contributor.authorPretorius, Isak S.
dc.date.accessioned2011-04-07T08:25:25Z
dc.date.available2011-04-07T08:25:25Z
dc.date.issued2002-01
dc.descriptionThe original publication is available at www.springerlink.com.
dc.descriptionIncludes bibliography.
dc.description.abstractA haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae alpha-amylase-encoding gene (LKA1). These transformants displayed significant differences in terms of the level of secretory expression of LKA1 under control of the PGK1 promoter and terminator, as well as their ability to produce and secrete the LKA1-encoded rawstarch-degrading alpha-amylase and to ferment starch. These results demonstrate the importance of the selection of appropriate host strains for yeast development pursuant to starch conversion into commercially important commodities via consolidated bioprocessing.en_ZA
dc.format.extentp. 651–656 : ill.
dc.identifier.citationGundllapalli Moses, S.B., Cordero Otero, R.R., La Grange, D.C., Van Rensburg, P., Pretorius, I.S. Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae. 2002. Biotechnology Letters, 651:651-656, www.springerlink.comen_ZA
dc.identifier.issn0141-5492 (Print Version)
dc.identifier.issn1573-6776 (Online Version)
dc.identifier.urihttp://hdl.handle.net/10019.1/8485
dc.language.isoen_ZAen_ZA
dc.publisherKluwer (now part of Springer)en_ZA
dc.rights.holderKluwer (now part of Springer)en_ZA
dc.subjectRecombinanten_ZA
dc.subjectHeterologousen_ZA
dc.subjectLipomycesen_ZA
dc.subjectLipomycesen_ZA
dc.subjectKononenkoaeen_ZA
dc.subjectAmylaseen_ZA
dc.subjectIndustrial yeasten_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.subjectStarchen_ZA
dc.subjectUtilisationen_ZA
dc.titleDifferent genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae alpha-amylase in industrial strains of Saccharomyces cerevisiaeen_ZA
dc.typeArticleen_ZA
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